HSPE1 (9B3) Monoclonal Antibody

$Lane 1: Mouse Liver; Probed with HSPE1 (9B3) Monoclonal Antibody (bsm-54399R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Liver; Probed with HSPE1 (9B3) Monoclonal Antibody (bsm-54399R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with HSPE1 (9B3) Monoclonal Antibody (bsm-54399R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with HSPE1 (9B3) Monoclonal Antibody (bsm-54399R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with HSPE1 (9B3) Monoclonal Antibody (bsm-54399R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with HSPE1 (9B3) Monoclonal Antibody (bsm-54399R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with HSPE1 (9B3) Monoclonal Antibody (bsm-54399R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with HSPE1 (9B3) Monoclonal Antibody (bsm-54399R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Kidney tissue incubated with HSPE1 (9B3) Monoclonal Antibody (bsm-54399R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Kidney tissue incubated with HSPE1 (9B3) Monoclonal Antibody (bsm-54399R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P
IP

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54399R
Product Name	HSPE1 (9B3) Monoclonal Antibody
Applications	WB, IHC-P, IP
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human Cpn10 aa 1-150.
Clonality	Monoclonal
Clone #	9B3
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	3336
Swiss Prot	P61604
Subcellular location	Mitochondrion
Synonyms	10 kDa heat shock protein mitochondrial; 10 kDa chaperonin; Chaperonin 10; Early-pregnancy factor; Hsp10; CPN10; EPF; HSPE1
Background	Co-chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp60, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable)
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IP	1-2ug