Cullin 2 (4C9) Monoclonal Antibody

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with Cullin 2 (4C9) Monoclonal Antibody (bsm-54381R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with Cullin 2 (4C9) Monoclonal Antibody (bsm-54381R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with Cullin 2 (4C9) Monoclonal Antibody (bsm-54381R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.$

Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with Cullin 2 (4C9) Monoclonal Antibody (bsm-54381R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.

$Lane 1: PC-3M Cells; Lane 2: K562 Cells; Probed with Cullin 2 (4C9) Monoclonal Antibody (bsm-54381R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: PC-3M Cells; Lane 2: K562 Cells; Probed with Cullin 2 (4C9) Monoclonal Antibody (bsm-54381R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
IHC-P
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54381R
Product Name	Cullin 2 (4C9) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human Cullin 2 aa 1-200.
Clonality	Monoclonal
Clone #	4C9
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	8453
Swiss Prot	Q13617
Subcellular location	Cytoplasm, Nucleus
Synonyms	Cullin-2; CUL-2; CUL2
Background	Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200