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Mib1 (1C8) Monoclonal Antibody

Applications

  • WB
  • IHC-P
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-54377R
Product Name Mib1 (1C8) Monoclonal Antibody
Applications WB, IHC-P, IF(IHC-P), IF(ICC)
Reactivity Human, Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant protein within N-terminal human Mib1.
Clonality Monoclonal
Clone # 1C8
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID 57534
Swiss Prot Q86YT6
Subcellular location Cytoplasm, Cell membrane, Cytoskeleton
Synonyms E3 ubiquitin-protein ligase MIB1; DAPK-interacting protein 1; Mind bomb homolog 1; RING-type E3 ubiquitin transferase MIB1; Zinc finger ZZ type with ankyrin repeat domain protein 2; DIP-1; MIB1; DIP1; KIAA1323; ZZANK2
Background E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis. Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200