SOCS2 (7G4) Monoclonal Antibody

$Lane 1: Human Placenta; Lane 2: SH-SY5Y Cells; Probed with SOCS2 (7G4) Monoclonal Antibody (bsm-54374R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Human Placenta; Lane 2: SH-SY5Y Cells; Probed with SOCS2 (7G4) Monoclonal Antibody (bsm-54374R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with SOCS2 (7G4) Monoclonal Antibody (bsm-54374R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with SOCS2 (7G4) Monoclonal Antibody (bsm-54374R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with SOCS2 (7G4) Monoclonal Antibody (bsm-54374R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with SOCS2 (7G4) Monoclonal Antibody (bsm-54374R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with SOCS2 (7G4) Monoclonal Antibody (bsm-54374R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with SOCS2 (7G4) Monoclonal Antibody (bsm-54374R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Applications

WB
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-54374R
Product Name	SOCS2 (7G4) Monoclonal Antibody
Applications	WB, IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human SOCS2 1-150aa.
Clonality	Monoclonal
Clone #	7G4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	8835
Swiss Prot	O14508
Subcellular location	Cytoplasm
Synonyms	Suppressor of cytokine signaling 2; Cytokine-inducible SH2 protein 2; STAT-induced STAT inhibitor 2; SOCS-2; CIS-2; SSI-2; SOCS2; CIS2; SSI2; STATI2
Background	SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200