IRAK2 (7A4) Monoclonal Antibody

Flow cytometric analysis of Jurkat cells with IRAK2 (7A4) Monoclonal Antibody (bsm-54364R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

$Paraformaldehyde-fixed and paraffin-embedded Human Heart tissue incubated with IRAK2 (7A4) Monoclonal Antibody (bsm-54364R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Heart tissue incubated with IRAK2 (7A4) Monoclonal Antibody (bsm-54364R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Prostate tissue incubated with IRAK2 (7A4) Monoclonal Antibody (bsm-54364R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Prostate tissue incubated with IRAK2 (7A4) Monoclonal Antibody (bsm-54364R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with IRAK2 (7A4) Monoclonal Antibody (bsm-54364R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with IRAK2 (7A4) Monoclonal Antibody (bsm-54364R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$IF(ICC) staining with IRAK2 (7A4) Monoclonal Antibody (bsm-54364R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with IRAK2 (7A4) Monoclonal Antibody (bsm-54364R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Lane 1: PC-3M Cells; Lane 2: A549 Cells; Lane 3: HepG2 Cells; Probed with IRAK2 (7A4) Monoclonal Antibody (bsm-54364R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: PC-3M Cells; Lane 2: A549 Cells; Lane 3: HepG2 Cells; Probed with IRAK2 (7A4) Monoclonal Antibody (bsm-54364R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-54364R
Product Name	IRAK2 (7A4) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human IRAK2 aa 1-200.
Clonality	Monoclonal
Clone #	7A4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	3656
Swiss Prot	O43187
Subcellular location	Cytoplasm, Nucleus
Synonyms	Interleukin-1 receptor-associated kinase-like 2; IRAK-2
Background	IRAK2 encodes the interleukin-1 receptor-associated kinase 2, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. IRAK2 is reported to participate in the IL1-induced upregulation of NF-kappaB. IRAK2 has been shown to interact with TRAF6 and Myd88. Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200