SFPQ (2C6) Monoclonal Antibody

$Lane 1: K562 Cells; Lane 2: Daudi Cells; Lane 3: Mouse Testis; Lane 4: Mouse Lung; Probed with SFPQ (2C6) Monoclonal Antibody (bsm-54249R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: K562 Cells; Lane 2: Daudi Cells; Lane 3: Mouse Testis; Lane 4: Mouse Lung; Probed with SFPQ (2C6) Monoclonal Antibody (bsm-54249R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with SFPQ (2C6) Monoclonal Antibody (bsm-54249R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with SFPQ (2C6) Monoclonal Antibody (bsm-54249R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with SFPQ (2C6) Monoclonal Antibody (bsm-54249R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with SFPQ (2C6) Monoclonal Antibody (bsm-54249R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with SFPQ (2C6) Monoclonal Antibody (bsm-54249R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with SFPQ (2C6) Monoclonal Antibody (bsm-54249R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with SFPQ (2C6) Monoclonal Antibody (bsm-54249R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with SFPQ (2C6) Monoclonal Antibody (bsm-54249R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with SFPQ (2C6) Monoclonal Antibody (bsm-54249R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with SFPQ (2C6) Monoclonal Antibody (bsm-54249R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54249R
Product Name	SFPQ (2C6) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within Human SFPQ aa 600 to the C-terminus
Clonality	Monoclonal
Clone #	2C6
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	6421
Swiss Prot	P23246
Subcellular location	Cytoplasm, Nucleus
Synonyms	Splicing factor proline- and glutamine-rich; 100 kDa DNA-pairing protein; DNA-binding p52/p100 complex 100 kDa subunit; Polypyrimidine tract-binding protein-associated-splicing factor; hPOMp100; PSF; PTB-associated-splicing factor; SFPQ.
Background	DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as a heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Involved in regulation of signal-induced alternative splicing. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400