GIT1 (4C1) Monoclonal Antibody

$Lane 1: A549 Cells; Probed with GIT1 (4C1) Monoclonal Antibody (bsm-54239R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: A549 Cells; Probed with GIT1 (4C1) Monoclonal Antibody (bsm-54239R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with GIT1 (4C1) Monoclonal Antibody (bsm-54239R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with GIT1 (4C1) Monoclonal Antibody (bsm-54239R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Pancreas tissue incubated with GIT1 (4C1) Monoclonal Antibody (bsm-54239R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Pancreas tissue incubated with GIT1 (4C1) Monoclonal Antibody (bsm-54239R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Fallopian Tubes tissue incubated with GIT1 (4C1) Monoclonal Antibody (bsm-54239R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Fallopian Tubes tissue incubated with GIT1 (4C1) Monoclonal Antibody (bsm-54239R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with GIT1 (4C1) Monoclonal Antibody (bsm-54239R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with GIT1 (4C1) Monoclonal Antibody (bsm-54239R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of A549 cells with GIT1 (4C1) Monoclonal Antibody (bsm-54239R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54239R
Product Name	GIT1 (4C1) Monoclonal Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within C-terminal human GIT1
Clonality	Monoclonal
Clone #	4C1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	28964
Swiss Prot	Q9Y2X7
Subcellular location	Cytoplasm
Synonyms	ARF GTPase-activating protein GIT1; Cool-associated and tyrosine-phosphorylated protein 1; G protein-coupled receptor kinase-interactor 1; GRK-interacting protein 1; ARF GAP GIT1; CAT-1; CAT1; GIT1.
Background	GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve SDCCAG3 and PTPN13.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400