NR0B1 (8C2) Monoclonal Antibody

$Lane 1: A549 Cells; Probed with NR0B1 (8C2) Monoclonal Antibody (bsm-54234R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: A549 Cells; Probed with NR0B1 (8C2) Monoclonal Antibody (bsm-54234R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with NR0B1 (8C2) Monoclonal Antibody (bsm-54234R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with NR0B1 (8C2) Monoclonal Antibody (bsm-54234R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with NR0B1 (8C2) Monoclonal Antibody (bsm-54234R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with NR0B1 (8C2) Monoclonal Antibody (bsm-54234R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with NR0B1 (8C2) Monoclonal Antibody (bsm-54234R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with NR0B1 (8C2) Monoclonal Antibody (bsm-54234R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Flow cytometric analysis of A549 cells with NR0B1 (8C2) Monoclonal Antibody (bsm-54234R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-54234R
Product Name	NR0B1 (8C2) Monoclonal Antibody
Applications	WB, FCM, IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human NR0B1/ Dax1 aa 1-150
Clonality	Monoclonal
Clone #	8C2
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	190
Swiss Prot	P51843
Subcellular location	Cytoplasm, Nucleus
Synonyms	Nuclear receptor subfamily 0 group B member 1; DSS-AHC critical region on the X chromosome protein 1; Nuclear receptor DAX-1; NR0B1; AHC; DAX1.
Background	Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(ICC)	1:50-200