MLKL (Ser345) Recombinant Antibody

$Lane 1: Mouse Spleen; Lane 2: Mouse Lung; Lane 3: Mouse Thymus; Probed with MLKL (Ser345) (7G4) Monoclonal Antibody (bsm-54104R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Spleen; Lane 2: Mouse Lung; Lane 3: Mouse Thymus; Probed with MLKL (Ser345) (7G4) Monoclonal Antibody (bsm-54104R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Lung tissue incubated with MLKL (Ser345) (7G4) Monoclonal Antibody (bsm-54104R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Lung tissue incubated with MLKL (Ser345) (7G4) Monoclonal Antibody (bsm-54104R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Liver tissue incubated with MLKL (Ser345) (7G4) Monoclonal Antibody (bsm-54104R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Liver tissue incubated with MLKL (Ser345) (7G4) Monoclonal Antibody (bsm-54104R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with MLKL (Ser345) (7G4) Monoclonal Antibody (bsm-54104R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with MLKL (Ser345) (7G4) Monoclonal Antibody (bsm-54104R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Spleen tissue incubated with MLKL (Ser345) (7G4) Monoclonal Antibody (bsm-54104R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Spleen tissue incubated with MLKL (Ser345) (7G4) Monoclonal Antibody (bsm-54104R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

Reactivity

Mouse

Overview
Catalog #	bsm-54104r-100ul
Product Name	MLKL (Ser345) Recombinant Antibody
Applications	WB, IP
Reactivity	Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic phospho-peptide corresponding to residues surrounding Ser345 of human MLKL
Modification Site	Ser345
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	74568
Swiss Prot	Q9D2Y4
Synonyms	Phospho-MLKL (S345); pMLKL Ser345; Mixed lineage kinase domain-like protein, Mlkl
Background	Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity.
Application Dilution
WB	1:300-5000
IP	1-2ug