TXNRD1 (3B7) Monoclonal Antibody

$Lane 1: Hela Cells; Lane 2: Human Liver; Probed with TXNRD1 (3B7) Monoclonal Antibody (bsm-54049R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Hela Cells; Lane 2: Human Liver; Probed with TXNRD1 (3B7) Monoclonal Antibody (bsm-54049R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with TXNRD1 (3B7) Monoclonal Antibody (bsm-54049R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with TXNRD1 (3B7) Monoclonal Antibody (bsm-54049R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with TXNRD1 (3B7) Monoclonal Antibody (bsm-54049R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with TXNRD1 (3B7) Monoclonal Antibody (bsm-54049R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with TXNRD1 (3B7) Monoclonal Antibody (bsm-54049R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with TXNRD1 (3B7) Monoclonal Antibody (bsm-54049R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of SH-SY5Y cells TXNRD1 (3B7) Monoclonal Antibody (bsm-54049R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54049R
Product Name	TXNRD1 (3B7) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Full length protein
Clonality	Monoclonal
Clone #	3B7
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store for 1 year at -20C from date of shipment. Store for 1 year at -20C from date of shipment.
Target
Gene ID	7296
Swiss Prot	Q16881
Synonyms	Thioredoxin reductase 1, cytoplasmic, Gene associated with retinoic and interferon-induced mortality 12 protein, KM-102-derived reductase-like factor, Thioredoxin reductase TR1, TR, GRIM-12, TXNRD1, GRIM12, KDRF
Background	Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200