Smooth muscle Myosin heavy chain 11 (1F11) Monoclonal Antibody

$IF(ICC) staining with Smooth muscle Myosin heavy chain 11 (1F11) Monoclonal Antibody (bsm-54033R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Smooth muscle Myosin heavy chain 11 (1F11) Monoclonal Antibody (bsm-54033R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Smooth muscle Myosin heavy chain 11 (1F11) Monoclonal Antibody (bsm-54033R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Smooth muscle Myosin heavy chain 11 (1F11) Monoclonal Antibody (bsm-54033R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human Jejunum tissue incubated with Smooth muscle Myosin heavy chain 11 (1F11) Monoclonal Antibody (bsm-54033R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Jejunum tissue incubated with Smooth muscle Myosin heavy chain 11 (1F11) Monoclonal Antibody (bsm-54033R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat Trachea tissue incubated with Smooth muscle Myosin heavy chain 11 (1F11) Monoclonal Antibody (bsm-54033R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Trachea tissue incubated with Smooth muscle Myosin heavy chain 11 (1F11) Monoclonal Antibody (bsm-54033R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with Smooth muscle Myosin heavy chain 11 (1F11) Monoclonal Antibody (bsm-54033R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with Smooth muscle Myosin heavy chain 11 (1F11) Monoclonal Antibody (bsm-54033R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Prostate tissue incubated with Smooth muscle Myosin heavy chain 11 (1F11) Monoclonal Antibody (bsm-54033R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Prostate tissue incubated with Smooth muscle Myosin heavy chain 11 (1F11) Monoclonal Antibody (bsm-54033R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Stomach Cancer tissue incubated with Smooth muscle Myosin heavy chain 11 (1F11) Monoclonal Antibody (bsm-54033R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Stomach Cancer tissue incubated with Smooth muscle Myosin heavy chain 11 (1F11) Monoclonal Antibody (bsm-54033R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Rat

Overview
Catalog #	bsm-54033r-100ul
Product Name	Smooth muscle Myosin heavy chain 11 (1F11) Monoclonal Antibody
Applications	WB, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	C terminal human smooth muscle Myosin heavy chain 11
Clonality	Monoclonal
Clone #	1F11
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	4629
Swiss Prot	P35749
Synonyms	Myosin-11, Myosin heavy chain 11, Myosin heavy chain, smooth muscle isoform, SMMHC, MYH11, KIAA0866
Background	The protein encoded by this gene is a smooth muscle myosin belonging to the myosin heavy chain family. The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. The gene encoding a human ortholog of rat NUDE1 is transcribed from the reverse strand of this gene, and its 3' end overlaps with that of the latter. The pericentric inversion of chromosome 16[inv(16)(p13q22)] produces a chimeric transcript that encodes a protein consisting of the first 165 residues from the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Alternative splicing generates isoforms that are differentially expressed, with ratios changing during muscle cell maturation. Alternatively spliced transcript variants encoding different isoforms have been identified. MYH11 is a smooth muscle myosin belonging to the myosin heavy chain family. It is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200