MINA53 (3C2) Monoclonal Antibody

$PC-12 cells lysates probed with MINA53 (3C2) Monoclonal Antibody (bsm-52981R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

PC-12 cells lysates probed with MINA53 (3C2) Monoclonal Antibody (bsm-52981R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
IF(ICC)

Reactivity

Human
Mouse

Overview
Catalog #	bsm-52981R
Product Name	MINA53 (3C2) Monoclonal Antibody
Applications	WB, IF(ICC)
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human MINA53 between 400 to the C-terminus
Clonality	Monoclonal
Clone #	3C2
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	84864
Swiss Prot	Q8IUF8
Synonyms	Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA, 60S ribosomal protein L27a histidine hydroxylase, Histone lysine demethylase MINA, MYC-induced nuclear antigen, Mineral dust-induced gene protein, Nucleolar protein 52, Ribosomal oxygenase MINA, ROX, MINA
Background	Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Is involved in the demethylation of trimethylated 'Lys-9' on histone H3 (H3K9me3), leading to an increase in ribosomal RNA expression. Also catalyzes the hydroxylation of 60S ribosomal protein L27a on 'His-39'. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200