CCR7 (1G8) Monoclonal Antibody

$Lane 1: MCF-7; Lane 2: PC-12; Lane 3: Daudi; Lane 4: Jurkat\r\nlysate; Probed with CCR7 (1G8) Monoclonal Antibody (bs-8554R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: MCF-7; Lane 2: PC-12; Lane 3: Daudi; Lane 4: Jurkat\r\nlysate; Probed with CCR7 (1G8) Monoclonal Antibody (bs-8554R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with CCR7 (1G8) Monoclonal Antibody (bsm-52274R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with CCR7 (1G8) Monoclonal Antibody (bsm-52274R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with CCR7 (1G8) Monoclonal Antibody (bsm-52274R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with CCR7 (1G8) Monoclonal Antibody (bsm-52274R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with CCR7 (1G8) Monoclonal Antibody (bsm-52274R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with CCR7 (1G8) Monoclonal Antibody (bsm-52274R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with CCR7 (1G8) Monoclonal Antibody (bsm-52274R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with CCR7 (1G8) Monoclonal Antibody (bsm-52274R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Lane 1: Mouse Thymus tissue lysates; Lane 2: Mouse Spleen tissue lysates; Lane 3: Mouse Lymph node tissue lysates; Lane 4: Mouse Lung tissue lysates ; Lane 5: Rat Thymus tissue lysates;Lane 6:Rat Spleen tissue lysates; Lane 7:Rat Lymph node tissue lysates; Lane 8: Rat Lung tissue lysates; Lane 9: Human Jurkat cell lysates; Lane 10: Human MCF-7 cell lysates; Lane 11: Human Hela cell lysates ; Lane 12: Human HL60 cell lysates probed with CCR7 (1G8) Monoclonal Antibody, Unconjugated (bsm-52274R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52274R
Product Name	CCR7 (1G8) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinanat human CCR7 between 1 to 100 amino acids.
Clonality	Monoclonal
Clone #	1G8
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1236
Swiss Prot	P32248
Subcellular location	Cell membrane
Synonyms	C-C chemokine receptor type 7; BLR2; CDw197; Epstein-Barr virus-induced G-protein coupled receptor 1; MIP-3 beta receptor; CD19; C-C CKR-7; CC-CKR-7; CCR-7; EBI1; EBV-induced G-protein coupled receptor 1
Background	CCR7 is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. Signals mediated by this receptor regulate T cell homeostasis in lymph nodes, and may also function in the activation and polarization of T cells, and in chronic inflammation pathogenesis. Alternative splicing of this gene results in multiple transcript variants.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200