YY1(Nuclear Loading Control) Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Monkey brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with YY1(Nuclear Loading Control) Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:25 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Monkey brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with YY1(Nuclear Loading Control) Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:25 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with YY1(Nuclear Loading Control) Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:25 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with YY1(Nuclear Loading Control) Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:25 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Zebrafish brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with YY1(Nuclear Loading Control) Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:25 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Zebrafish brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with YY1(Nuclear Loading Control) Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:25 overnight at 4\u00b0C, DAB staining.

$Lane 1: Daudi cell lysates; Lane 2: U251 cell lysates probed with YY1 Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Daudi cell lysates; Lane 2: U251 cell lysates probed with YY1 Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: 293 cell lysates; Lane 2:C6 cell lysates; Lane 3: Hela cell lysates; Lane 4: U-251MG cell lysates probed with YY1 Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: 293 cell lysates; Lane 2:C6 cell lysates; Lane 3: Hela cell lysates; Lane 4: U-251MG cell lysates probed with YY1 Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F

Reactivity

Others

Predicted Reactivity

Human
Rat
Monkey

Overview
Catalog #	bsm-51566M
Product Name	YY1(Nuclear Loading Control) Monoclonal Antibody
Applications	WB, IHC-P, IHC-F
Reactivity	Others
Predicted Reactivity	Human, Rat, Monkey
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human YY1 (His-tagged).
Clonality	Monoclonal
Clone #	G23F3
Isotype	IgG2a
Concentration	0.5ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7528
Swiss Prot	P25490
Subcellular location	Nucleus
Synonyms	Yin Yang 1; YY1 transcription factor; CF1; Delta; Delta transcription factor; NF E1; NFE1; Transcriptional repressor protein YY1; UCR motif DNA binding protein; UCRBP; Yin and yang 1; Yin Yang 1; Ying Yang 1; YY 1; YY 1 transcription factor; TYY1_HUMAN.
Background	Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions). Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; proposed to target the INO80 complex to YY1-responsive elements.
Application Dilution
WB	=1:500-1000ÔºåIHC-P=1:25-50ÔºåIHC-F=1:25-50Ôºå
IHC-P	1:200-400
IHC-F	1:100-500