USP5 (13C4) Monoclonal Antibody

$Lane 1: mouse brain lysates; Lane 2: PC-3 Cell lysates; Lane 3: rat liver Cell lysates; Lane 4: U-251 MG Cell lysates; Lane 5: rat brain Cell lysates; probed with USP5 (13C4)) Monoclonal Antibody, unconjugated (bsm-51404M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: mouse brain lysates; Lane 2: PC-3 Cell lysates; Lane 3: rat liver Cell lysates; Lane 4: U-251 MG Cell lysates; Lane 5: rat brain Cell lysates; probed with USP5 (13C4)) Monoclonal Antibody, unconjugated (bsm-51404M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-51404M
Product Name	USP5 (13C4) Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This USP5 antibody is generated from a mouse immunized with a recombination protein from the human region of human USP5.
Clonality	Monoclonal
Clone #	13C4
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	8078
Swiss Prot	P45974
Synonyms	ISOT; Ubiquitin carboxyl-terminal hydrolase 5; Deubiquitinating enzyme 5; Isopeptidase T; Ubiquitin thioesterase 5; Ubiquitin-specific-processing protease 5; USP5; USP 5; USP-5
Background	Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
Application Dilution
WB	1:300-5000