BCL2L1 (4C4) Monoclonal Antibody

$Lane 1: HepG2 Cell lysates; Lane 2: MDA-MB-231 Cell lysates; Lane 3: NIH\/3T3 Cell lysates; Lane 4: PC-12 Cell lysates; probed with BCL2L1 (804CT19.1.4) Monoclonal Antibody, unconjugated (bsm-51229M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: HepG2 Cell lysates; Lane 2: MDA-MB-231 Cell lysates; Lane 3: NIH\/3T3 Cell lysates; Lane 4: PC-12 Cell lysates; probed with BCL2L1 (804CT19.1.4) Monoclonal Antibody, unconjugated (bsm-51229M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-51229M
Product Name	BCL2L1 (4C4) Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Purified His-tagged BCL2L1 protein was used to produced this monoclonal antibody.
Clonality	Monoclonal
Clone #	4C4
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	598
Swiss Prot	Q07817
Synonyms	BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S; Bcl-2-like protein 1; Bcl2-L-1; Apoptosis regulator Bcl-X; BCL2L1
Background	Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F(1)F(0) activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785). Isoform Bcl-X(S) promotes apoptosis.
Application Dilution
WB	1:300-5000