MYOG (4C1) Monoclonal Antibody

$Mouse heart lysates; probed with MYOG (4C1) Monoclonal Antibody, unconjugated (bsm-51095M) overnight at 4\u00b0C followed by a conjugated secondary antibody.$

Mouse heart lysates; probed with MYOG (4C1) Monoclonal Antibody, unconjugated (bsm-51095M) overnight at 4\u00b0C followed by a conjugated secondary antibody.

Applications

Reactivity

Mouse

Overview
Catalog #	bsm-51095M
Product Name	MYOG (4C1) Monoclonal Antibody
Applications	WB, FCM
Reactivity	Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This MYOG antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 30-58 amino acids from the N-terminal region of human MYOG.
Clonality	Monoclonal
Clone #	4C1
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	4656
Swiss Prot	P15173
Synonyms	MYF4; myf-4; bHLHc3; Myogenin; Class C basic helix-loop-helix protein 3; Myogenic factor 4; MYOG
Background	Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts (By similarity).
Application Dilution
WB	1:300-5000
FCM	1:20-100