CD90/Thy-1 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD90\/Thy-1) Monoclonal Antibody, Unconjugated (bsm-43534M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD90\/Thy-1) Monoclonal Antibody, Unconjugated (bsm-43534M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

$Lane 1: Recombinant Human Thy-1 protein, C-His (HEK293) probed with CD90\/Thy-1 Monoclonal Antibody, Unconjugated (bsm-43534M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Recombinant Human Thy-1 protein, C-His (HEK293) probed with CD90\/Thy-1 Monoclonal Antibody, Unconjugated (bsm-43534M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
FCM
IHC-P

Reactivity

Human
Rat

Overview
Catalog #	bsm-43534M
Product Name	CD90/Thy-1 Monoclonal Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant Human Thy-1 protein, C-His (HEK293)
Clonality	Monoclonal
Clone #	-
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	24832
Swiss Prot	P01830
Subcellular location	Cell membrane
Synonyms	CD90 / Thy1; CD7; CD90 antigen; CDw90; FLJ33325; MGC128895; T25; Theta antigen; Thy-1; Thy 1; Thy 1 cell surface antigen; Thy-1 membrane glycoprotein; Thy 1 membrane glycoprotein precursor; Thy 1.2; Thy-1 T-cell antigen; Thy1 antigen; Thy1 T cell antigen; Thy1.1; Thy1.2; Thymus cell antigen 1, theta; THY1_RAT; THY1_HUMAN.
Application Dilution
WB	=1:500-2000
FCM	FCM=1:100
IHC-P	IHC-P=1:100-500