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SARS-CoV-2 (2019-nCoV) Spike Neutralizing Monoclonal Antibody

Applications

  • ELISA

Reactivity

  • SARS-CoV-2
Overview
Catalog # bsm-41636M
Product Name SARS-CoV-2 (2019-nCoV) Spike Neutralizing Monoclonal Antibody
Applications ELISA
Reactivity SARS-CoV-2
Specifications
Conjugation Unconjugated
Host Mouse
Source Recombinant SARS-CoV-2 Spike S1 Protein
Immunogen Range 14-685/1213
Clonality Monoclonal
Clone # 7H3
Isotype IgG3
Concentration 1mg/ml
Purification affinity purified by Protein A
Storage Buffer 0.01M PBS(pH7.4)
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 43740568
Swiss Prot P0DTC2
Synonyms SARS-CoV-2 spike protein; 2019-nCOV Spike protein; Spike glycoprotein; Spike protein S1; Surface glycoprotein; Spike protein RBD; SPIKE_SARS2.
Background The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
Application Dilution
ELISA 1:500-1000