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IKB alpha Monoclonal Antibody, PerCP-Cy5.5 Conjugated

Applications

  • WB

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-33441M-PerCP-Cy5.5
Product Name IKB alpha Monoclonal Antibody, PerCP-Cy5.5 Conjugated
Applications WB
Reactivity Human, Mouse, Rat
Specifications
Conjugation PerCP-Cy5.5
Host Mouse
Source Recombinant human IKB alpha Protein
Immunogen Range 1-77/314
Clonality Monoclonal
Clone # 2A5
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID P25963
Swiss Prot 4792
Subcellular location Cytoplasm, Nucleus
Synonyms NFKBIA; Inhibitor of KB alpha; I kappa B alpha; I(Kappa)B(alpha); IkappaBalpha; IKBA; IKBalpha; MAD 3; MAD3; Major histocompatibility complex enhancer binding protein MAD3; NF kappa B inhibitor alpha; NFKBI; NFKBIA; Nuclear factor of kappa light chain gene enhancer in B cells; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha; IKBA_HUMAN.
Background Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting.
Application Dilution
WB 1:300-5000