ERK1 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (human colon ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ERK1) Monoclonal Antibody, Unconjugated (bsm-33335M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human colon ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ERK1) Monoclonal Antibody, Unconjugated (bsm-33335M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

$Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ERK1) Monoclonal Antibody, Unconjugated (bsm-33335M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ERK1) Monoclonal Antibody, Unconjugated (bsm-33335M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Applications

IHC-P
IHC-F
IF
ICC

Reactivity

Human

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bsm-33335M
Product Name	ERK1 Monoclonal Antibody
Applications	IHC-P, IHC-F, IF, ICC
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human ERK1 full length
Clonality	Monoclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5595
Swiss Prot	Q63844
Subcellular location	Nucleus
Synonyms	ERK; ERK-1; ERT 2; ERT2; Extracellular Signal Regulated Kinase 1; Extracellular signal related kinase 1; Extracellular signal-regulated kinase 1; HGNC6877; HS44KDAP; HUMKER1A; Insulin Stimulated MAP2 Kinase; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP Kinase; MAP kinase isoform p44; MAPK 1; MAPK 3; MAPK; MAPK1; Mapk3; MGC20180; Microtubule Associated Protein 2 Kinase; Microtubule-associated protein 2 kinase; Mitogen Activated Protein Kinase 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 3; MK03_HUMAN; OTTHUMP00000174538; OTTHUMP00000174541; p44 ERK1; p44 MAPK; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM 3; PRKM3; Protein Kinase Mitogen Activated 3.
Application Dilution
IHC-P	=1:100-500
IHC-F	IHC-F=1:100-500
IF	IF=1:100-500
ICC	ICC=1:100-500