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AmCyan-Tag (5F9) Monoclonal Antibody, RBITC Conjugated

Applications

  • WB

Reactivity

  • Others
Overview
Catalog # bsm-33184M-RBITC
Product Name AmCyan-Tag (5F9) Monoclonal Antibody, RBITC Conjugated
Applications WB
Reactivity Others
Specifications
Conjugation RBITC
Host Mouse
Source Recombinant AmCyan
Clonality Monoclonal
Clone # 5F9
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein G.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Background AmCyan1 is a human codon-optimized variant of the wild-type Anemonia majano cyan fluorescent protein (AmCyan) that exhibits enhanced emission characteristics (excitation and emission maxima: 458nm and 489nm, respectively). DD-AmCyan1 is a modified version of AmCyan1 that is tagged on its N-terminus with the ProteoTuner destabilization domain (DD). The presence of this destabilization domain causes rapid, proteasomal degradation of the fluorescent fusion protein; however, when the membrane permeant ligand Shield1 is added to the medium, it binds to the destabilization domain and protects the fusion protein from degradation.
Application Dilution
WB 1:300-5000