Aquaporin 4 monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Aquaporin 4 Monoclonal Antibody, Unconjugated (bsm-33068M) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Aquaporin 4 Monoclonal Antibody, Unconjugated (bsm-33068M) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Aquaporin 4 Monoclonal Antibody, Unconjugated (bsm-33068M) at 1:800 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Aquaporin 4 Monoclonal Antibody, Unconjugated (bsm-33068M) at 1:800 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Aquaporin 4 Monoclonal Antibody, Unconjugated (bsm-33068M) at 1:800 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Aquaporin 4 Monoclonal Antibody, Unconjugated (bsm-33068M) at 1:800 overnight at 4\u00b0C, DAB staining.

Applications

IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-33068M
Product Name	Aquaporin 4 monoclonal Antibody
Applications	IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human Aquaporin 4 Protein
Clonality	Monoclonal
Clone #	6G1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	361
Swiss Prot	P55087
Subcellular location	Cell membrane
Synonyms	aquaporin 4; WCH4; Mercurial-insensitive water channel; MIWC; AQP 4; AQP4; Aquaporin4; HMIWC 2; HMIWC2; Mercurial insensitive water channel; MGC22454; MIWC; AQP4_HUMAN; Aquaporin-4; AQP-4.
Background	This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Application Dilution
IHC-P	1:200-400