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p53 (8D6) Monoclonal Antibody, Cy5.5 Conjugated

Applications

  • WB

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-33058M-Cy5.5
Product Name p53 (8D6) Monoclonal Antibody, Cy5.5 Conjugated
Applications WB
Reactivity Human, Mouse
Specifications
Conjugation Cy5.5
Host Mouse
Source KLH conjugated synthetic peptide derived from human p53
Clonality Monoclonal
Clone # 8D6
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein G.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7157
Swiss Prot P04637
Synonyms Cellular tumor antigen p53; TP53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53; P53
Background Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).
Application Dilution
WB 1:300-5000