MARCH7 Polyclonal Antibody

$MCF-7 Cell lysates probed with MARCH7 Polyclonal Antibody, unconjugated (bs-9341R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

MCF-7 Cell lysates probed with MARCH7 Polyclonal Antibody, unconjugated (bs-9341R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
IHC-P
IF(IHC-P)
IP

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-9341R
Product Name	MARCH7 Polyclonal Antibody
Applications	WB, IHC-P, IF(IHC-P), IP
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MARCH7
Immunogen Range	31-130/704
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	64844
Swiss Prot	Q9H992
Synonyms	AXO; AXOT; Axotrophin; E3 ubiquitin-protein ligase MARCH7; MARCH VII; MARCH-VII; March7; MARH7_HUMAN; Membrane associated RING CH protein VII; Membrane associated ring finger C3HC4 7 E3 ubiquitin protein ligase; membrane associated ring finger C3HC4 7; Membrane associated RING finger protein 7; Membrane-associated RING finger protein 7; Membrane-associated RING-CH protein VII; RING finger protein 177; RNF177.
Background	Axotrophin is a stem cell gene that encodes a protein which is involved in T lymphocyte regulation (especially in regulating the proliferation) and leukemia inhibitory factor (LIF) release. LIF is a neuropoietic cytokine that is important for stem cell regulation and thymocyte stimulation. Both Axotrophin and LIF are linked to transplantation intolerance. Axotrophin is also involved in corpus callosum differentiation and may play a role in glial cell line-derived neurotrophic factor (GDNF)-dependent sensory neuron survival in the substantia gelatinosa of the adult spinal cord. Axotrophin is primarily expressed in the hippocampus, cortex, purkinje and granule cells of the cerebellum.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IP	1-2ug