LRCH1 Polyclonal Antibody

Spleen lysates probed with LRCH1 Polyclonal Antibody, Unconjugated (bs-9327R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LRCH1 Polyclonal Antibody, Unconjugated (bs-9327R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LRCH1 Polyclonal Antibody, Unconjugated (bs-9327R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LRCH1 Polyclonal Antibody, Unconjugated (bs-9327R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LRCH1 Polyclonal Antibody, Unconjugated (bs-9327R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LRCH1 Polyclonal Antibody, Unconjugated (bs-9327R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LRCH1 Polyclonal Antibody, Unconjugated (bs-9327R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LRCH1 Polyclonal Antibody, Unconjugated (bs-9327R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LRCH1 Polyclonal Antibody, Unconjugated (bs-9327R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

Applications

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Cow
Sheep
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-9327r-100ul
Product Name	LRCH1 Polyclonal Antibody
Applications	IHC
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human LRCH1
Immunogen Range	221-320/728
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23143
Swiss Prot	Q9Y2L9
Subcellular location	Cytoplasm
Synonyms	Calponin homology domain-containing protein 1; CHDC1; KIAA1016; Leucine-rich repeat and calponin homology domain-containing protein 1 antibody Lrch1; LRCH1_HUMAN; Neuronal protein 81; NP81.
Background	Members of the leucine-rich repeat family include LRCH1, LRCH2, LRCH3 and LRCH4. All family members contain one calponin-homology domain and nine leucine-rich repeats. The gene encoding LRCH1, also known as Neuronal protein 81 (NP81), has been suggested to be a possible gene locus for susceptibility to osetoarthritis, especially in women, though studies have shown conflicting results. LRCH1 is a 728 amino acid protein that is expressed at highest levels in cerebellum, with lower levels of expression in spinal cord, ovary, kidney and amygdala. In pigs, the gene encoding LRCH1 is one of 22 genes that is associated with leg and body conformation traits that affect productivity and health. There are two isoforms of LRCH1 that are expressed as a result of alternative splicing events.
Application Dilution
IHC	1:100-500