RNF149 Polyclonal Antibody

Mouse stomach lysates probed with Anti-RNF149 Polyclonal Antibody (bs-9228R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C

$Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with RNF149 Polyclonal Antibody, Unconjugated (bs-9228R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with RNF149 Polyclonal Antibody, Unconjugated (bs-9228R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA

Reactivity

Mouse
Rat

Predicted Reactivity

Human

Overview
Catalog #	bs-9228R
Product Name	RNF149 Polyclonal Antibody
Applications	WB, ELISA
Reactivity	Mouse, Rat
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human RNF149
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	284996
Swiss Prot	Q8NC42
Subcellular location	Cell membrane
Synonyms	DNA polymerase-transactivated protein 2; E3 ubiquitin-protein ligase RNF149; RING finger protein 149; RN149_HUMAN; Rnf149.
Application Dilution
WB	=1:500-2000
ELISA	ELISA=1:5000-10000