KIFC1 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti KIFC1 Polyclonal Antibody, Unconjugated (bs-7805R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti KIFC1 Polyclonal Antibody, Unconjugated (bs-7805R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$L1 human colon carcinoma lysates L2 rat brain lysates probed with Anti KIFC1 Polyclonal Antibody, Unconjugated (bs-7805R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 74kD. Observed band size:74kD.\\n$

L1 human colon carcinoma lysates L2 rat brain lysates probed with Anti KIFC1 Polyclonal Antibody, Unconjugated (bs-7805R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 74kD. Observed band size:74kD.\\n

$Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (KIFC1) Polyclonal Antibody, Unconjugated (bs-7805R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (KIFC1) Polyclonal Antibody, Unconjugated (bs-7805R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human
Mouse
Rat
Bovine
Pig

Overview
Catalog #	bs-7805R
Product Name	KIFC1 Polyclonal Antibody
Applications	WB, IHC-P, IF(IHC-P)
Reactivity	Human, Mouse, Rat, Bovine, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human KIFC1
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	3833
Synonyms	HSET KNSL2; K C1; KC 1; KC1; KC1_HUMAN; Kinesin family member C1; Kinesin like 2; Kinesin like protein 2; Kinesin like protein KC1; Kinesin related protein HSET; Kinesin-like protein 2; Kinesin-like protein KC1; Kinesin-related protein HSET; KNSL 2; KNSL2; Mitotic kinesin like protein 2; MKLP 2;
Background	The kinesins constitute a large family of microtubule-dependent motor proteins, which are responsible for the distribution of numerous organelles, vesicles and macromolecular complexes throughout the cell. Individual kinesin members play crucial roles in cell division, intracellular transport and membrane trafficking events including endocytosis and transcytosis. KIFC1 is a 673 amino acid protein that belongs to the kinesin-like family of proteins. KIFC1 localizes to the nucleus and contains a C-terminal kinesin-motor domain. Functioning as a minus-end directed microtubule-dependent motor, KIFC1 works together with NuMA and cytoplasmic Dynein to organize microtubule minus ends at spindle poles. HeLa cells deficient in KIFC1 exhibit multipolar mitotic spindles, suggesting that KIFC1 is essential for bipolar spindle formation.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200

Applications

Reactivity

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