Cyclophilin F Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cyclophilin F Polyclonal Antibody, Unconjugated (bs-7624R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cyclophilin F Polyclonal Antibody, Unconjugated (bs-7624R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Rat

Predicted Reactivity

Mouse
Cow
Pig
Chicken

Overview
Catalog #	bs-7624R
Product Name	Cyclophilin F Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Rat
Predicted Reactivity	Mouse, Cow, Pig, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Cyclophilin F
Immunogen Range	31-130/207
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10105
Swiss Prot	P30405
Subcellular location	Cytoplasm
Synonyms	Cyclophilin 3; Cyclophilin D; Cyclophilin F; Cyp D; CYP3; CypD; EC 5.2.1.8; hCyP3; Peptidyl prolyl cis trans isomerase, mitochondral; Peptidylprolyl isomerase F (cyclophilin F); Peptidylprolyl isomerase F; PPIase; PPIF; Rotamase.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200