Overview |
bs-70724r-100ul |
WASP / N-WASP Antibody |
WB |
This antibody detects a 63 kDa* protein corresponding to the molecular mass of WASP on SDS-PAGE immunoblots of Jurkat cell lysate. In rat brain, A431, and SKN-SH cells, this antibody detects a 65 kDa* protein corresponding to the molecular mass of N-WASP. |
Human, Mouse, Rat |
Specifications |
Unconjugated |
Rabbit |
A synthetic peptide (coupled to KLH) corresponding to a region near the C-terminus of human WASP. This sequence is highly homologous with a similar region in human N-WASP. |
Polyclonal |
IgG |
Antigen Affinity purification |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
Target |
O00401 |
Neural Wiskott-Aldrich syndrome protein, WASL, WASP |
Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Serine and tyrosine phosphorylation regulates the activity of both proteins. WASP is observed as a 63 kDa protein in hematopoietic cells, while N-WASP is observed as a 65 kDa in many tissues, especially brain. |
Application Dilution |
WB |
1:300-5000 |