| Overview |
| bs-70654r-100ul |
| Semaphorin-3F (C-terminal region) Antibody |
| WB |
| The antibody detects a 95 kDa* protein corresponding to the apparent molecular mass of Sema-3F on SDS-PAGE immunoblots of adult mouse brain and liver. This sequence is highly conserved in rat and mouse Sema-3F, and has low homology to other semaphorin family members. |
| Human, Mouse, Rat |
| Specifications |
| Unconjugated |
| Rabbit |
| Semaphorin 3F synthetic peptide (coupled to carrier protein) corresponds to amino acids from the C-terminal region of human Sema-3F. |
| Polyclonal |
| IgG |
| Antigen Affinity purification |
| PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target |
| Q13275 |
| One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that have been implicated in neuron development, vascular disease, and tumor progression. There are eight classes of semaphorin genes, all of which are characterized by a conserved 500 amino acid, cystine-rich Sema domain. Semaphorin 3F (Sema-3F) is a class III secreted semaphorin that binds with high affinity to Neuropilin-2, and low affinity to Neuropilin-1. During peripheral and central nervous system development, Sema-3F has critical roles in axon guidance and dendrite outgrowth. Sema-3F has also been shown to inhibit angiogenesis by manipulating VEGFR function and decreasing blood vessel density. In cancers, Sema-3F induces a poorly vascularized, encapsulated, non-metastatic phenotype through chemorepulsion of endothelial cells in melanoma, while Sema-3F disrupts intercellular contacts of MCF7 breast cancer cells through delocalization of E-cadherin and β-catenin. Thus, Sema-3F may have important roles in axon guidance, angiogenesis, and tumor progression |
| Application Dilution |
| WB |
1:300-5000 |
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