EGFR (Ser-967), Phosphospecific Antibody

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-70475r-100ul
Product Name	EGFR (Ser-967), Phosphospecific Antibody
Applications	WB
Specificity	This antibody was cross-adsorbed to unphosphorylated EGFR (Ser-967) coupled to agarose then Affinity purification using phospho-EGFR (Ser-967) peptide (without carrier). The antibody detects a 180 kDa* protein on SDS-PAGE immunoblots of human A431 cells treated with Calyculin A, and does not detect this band after lambda phosphatase treatment. This human EGFR sequence has high homology to rat and mouse EGFR, and low homology to other ErbB family members.
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Phospho-EGFR (Ser-967) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 967 of human EGFR (ErbB-1).
Modification Site	Ser-967
Clonality	Polyclonal
Isotype	IgG
Purification	Antigen Affinity purification
Storage Buffer	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Swiss Prot	P00533
Synonyms	EGF, Epidermal, ErbB
Background	The epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein with an extracellular ligand-binding domain and a cytoplasmic domain with intrinsic tyrosine kinase activity. The cytoplasmic domain has a C-terminal region with multiple autophosphorylation sites (Tyr-992, 1068, 1086, 1148, and 1173). These sites are important for downstream signaling and rapid internalization. In addition, EGFR activation leads to c-Src mediated phosphorylation of Tyr-845 and Tyr-1101. The former site is required for mitogenic responses to EGFR activation, while the latter may be an SH2 binding site. Phosphorylation of EGFR on serine and threonine residues is thought to represent a mechanism for regulation of receptor kinase activity and internalization. These sites include a PKC site (Thr-654), CAMKII sites (Ser-1046, 1047, 1057, and 1142), and constitutively phosphorylated sites (Ser-967 and Ser-1002). Thus, the regulation of EGFR activity involves a complex series of phosphorylation events at multiple sites throughout the intracellular portion of the receptor.
Application Dilution
WB	1:300-5000