| Overview |
| bs-70426r-100ul |
| Coronin-1B (C-terminus) Antibody |
| WB |
| The antibody detects a 60 kDa* band corresponding to Coronin-1B on SDS-PAGE immunoblots of human A431 and HeLa, mouse brain, and rabbit spleen fibroblasts. This sequence has high homology to the conserved site in rat and mouse coronin-1B, and less than 50% homology to similar regions in other coronins. |
| Human, Mouse, Rat |
| Specifications |
| Unconjugated |
| Rabbit |
| Coronin-1B (C-terminus) synthetic peptide (coupled to carrier protein) corresponds to amino acids at the C-terminus of human coronin-1B. |
| Polyclonal |
| IgG |
| Antigen Affinity purification |
| PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target |
| Q9BR76 |
| Coronin2, Coro1B |
| Coronins are highly-conserved F-actin binding proteins that play important roles in lamellipodial protrusion during various types of cell motility. In yeast, coronins regulate cytoskeletal changes through inhibition of Arp2/3 complex. Human coronins have been classified in three subgroups type I (coronin-1A, -1B, -1C), type II (coronin-2A, -2B), and type III (coronin-7). These coronins have at least one large b-propeller region that mediates protein-protein interactions and type I and II coronins have coiled-coil regions involved in oligiomerization. Coronin-1B is ubiquitously expressed and localizes to the leading edge of cell protrusions in migrating fibroblasts. Both Coronin-1B and Coronin-1A interaction with Arp2/3 complex may be regulated by phosphorylation. PKC phosphorylates the N-terminus at Ser-2 , and this phosphorylation reduces interactions with Arp2/3 leading to diminshed cell motility. |
| Application Dilution |
| WB |
1:300-5000 |
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