N-Cadherin (C-terminal region) Antibody

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-70364r-100ul
Product Name	N-Cadherin (C-terminal region) Antibody
Applications	WB, IP
Specificity	The purified antibody detects a band at 130 kDa* in western blots of human endothelial cells and mouse brain tissue, and does not cross-react with E-cadherin. The antibody also works for ELISA, immunoprecipitation, and immunocytochemistry. This sequence is conserved in rat and mouse N-cadherin, and has three amino acid differences from the conserved region in R-cadherin.
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	unphosphorylated N-Cadherin synthetic peptide (coupled to carrier protein) corresponding to amino acids from the C-terminal region of human N-cadherin.
Clonality	Polyclonal
Isotype	IgG
Purification	Antigen Affinity purification
Storage Buffer	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Swiss Prot	P19022
Synonyms	Cadherin-2, Neural-Cadherin, CD325
Background	Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 can disrupt cadherin binding to β-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.
Application Dilution
WB	1:300-5000
IP	1-2ug