| Overview |
| bs-70298R |
| Tryptophan Hydroxylase (Ser19) Antibody |
| WB |
| Specific for endogenous levels of the ~55 kDa tryptophan hydroxylase protein phosphorylated at Ser19 . |
| Mouse, Rat |
| Bovine, Zebrafish |
| Specifications |
| Unconjugated |
| Rabbit |
| Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser19 of rat tryptophan hydroxylase 2 (TPH2), conjugated to keyhole limpet hemocyanin (KLH). |
| Ser19 |
| Polyclonal |
| IgG |
| Lot Dependent |
| Antigen Affinity purification from Pooled whole antiserum |
| 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target |
| 317675 |
| Q8CGU9 |
| MGC138871 antibody, ADHD7 antibody, FLJ37295 antibody, MGC138872 antibody, Neuronal tryptophan hydroxylase antibody, NTPH antibody, TPH 2 antibody, Tph2 antibody, TPH2 antibody, TPH2_HUMAN antibody, Tryptophan 5-hydroxylase 2 antibody, Tryptophan 5-monooxygenase 2 antibody, Tryptophan hydroxylase 2 antibody |
| Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser-19 which lies within the regulatory domain of TPH2 (McKinney et al., 2005). |
| Application Dilution |
| WB |
1:300-5000 |
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