Troponin I (cardiac) Ser150 Antibody

Applications

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Non-Human Primate

Overview
Catalog #	bs-70295r-100ul
Product Name	Troponin I (cardiac) Ser150 Antibody
Applications	WB
Specificity	Specific for endogenous levels of the ~25 kDa cardiac troponin I protein phosphorylated at Ser150. Immunolabeling is greatly decreased with λ-phosphatase treatment.
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Non-Human Primate
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser150 of mouse troponin I, cardiac (cTnI), conjugated to keyhole limpet hemocyanin (KLH).
Modification Site	Ser150
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot Dependent
Purification	Antigen Affinity purification from Pooled whole antiserum
Storage Buffer	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Gene ID	21954
Swiss Prot	P48787
Synonyms	cardiac muscle antibody, Cardiac troponin I antibody, Cardiac Troponin I antibody, cardiomyopathy dilated 2A (autosomal recessive) antibody, Cardiomyopathy familial hypertrophic 7 included antibody, CMD1FF antibody, CMD2A antibody, CMH7 antibody, cTnI antibody, Familial hypertrophic cardiomyopathy 7 antibody, MGC116817 antibody, RCM1 antibody, Tn1 antibody, Tni antibody, TNN I3 antibody, TNNC 1 antibody, TNNC1 antibody, TNNI3 antibody, TNNI3_HUMAN antibody, Troponin I antibody, Troponin I cardiac antibody, Troponin I cardiac muscle antibody, Troponin I cardiac muscle isoform antibody, Troponin I type 3 cardiac antibody, troponin I cardiac 3 antibody, TroponinI antibody, Troponin I type 3 (cardiac) antibody
Background	Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle. cTnI is phosphorylated by protein kinase C and protein kinase A at Ser-23/24 (Noland et al, 1995) and is phosphorylated by AMPK at Ser-23 and Ser-150 (Solis et al, 2011). Evidence suggests that AMPK, a critical regulator of cardiac energetics, prefers phosphorylating Ser-150 over Ser-23, and may play a role in regulating energy consumption through altering the phosphorylation status of cTnI (Solis et al., 2011).
Application Dilution
WB	1:300-5000