TFAM (Transcription Factor A, mitochondrial) Antibody

Applications

Reactivity

Human

Overview
Catalog #	bs-70282r-100ul
Product Name	TFAM (Transcription Factor A, mitochondrial) Antibody
Applications	WB
Specificity	Specific for endogenous levels of the ~24 kDa TFAM protein. The antibody has been directly tested for reactivity in human. Shows lesser reactivity to rat and mouse due to some sequence homology, but our catalog # 2001-TFAM is recommended for use in rodent.
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Native recombinant human TFAM protein with c-terminal 6-his tag.
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot Dependent
Purification	Neat whole antiserum
Storage Buffer	Neat whole antiserum
Storage Condition	Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Target
Gene ID	7019
Swiss Prot	Q00059
Synonyms	Transcription factor 6-like 1 antibody, Mitochondrial transcription factor 1 antibody, mitochondrial transcription factor A antibody, MtTF1 antibody, mtTFA antibody, TCF 6 antibody, TCF-6 antibody, TCF6 antibody, TCF6L1 antibody, TCF6L2 antibody, TCF6L3 antibody, TFAM antibody, TFAM_HUMAN antibody, Transcription factor 6 antibody, Transcription factor 6 like 2 (mitochondrial transcription factor) antibody, Transcription factor 6 like 2 antibody, Transcription factor 6-like 2 antibody, transcription factor 6-like 3 antibody, Transcription factor A mitochondrial antibody, Transcription factor A mitochondrial antibody, Transcription factor A mitochondrial precursor antibody
Background	Mitochondrial Transcription Factor A (TFAM) is a key activator of mitochondrial (mt) DNA transcription as well as a participant in mitochondrial genome replication. mtDNA is highly susceptible to oxidative stress leading to mitochondrial dysfunction. Overexpression of TFAM has been implicated in the amelioration of age dependent impairment of brain functions through the prevention of oxidative stress and mitochondrial dysfunction in microglia (Hayashi et al., 2008). More recently, TFAM overexpression has been shown to potentially reduce oxidative stress in motor neurons and delay onset of amyotrophic lateral sclerosis (ALS) in ALS model mice (Morimoto et al., 2012).
Application Dilution
WB	1:300-5000