TDP43 Antibody

Applications

Reactivity

Human
Rat

Overview
Catalog #	bs-70281r-100ul
Product Name	TDP43 Antibody
Applications	WB, IHC
Specificity	Specific for endogenous levels of the ~43 kDa TDP-43 protein in Western blots of rat brain lysate. Expected molecular weight is dependent upon the TDP-43 species present in sample (full-length vs. truncated TDP-43). Under non-denaturing conditions (for example, by IHC), this antibody detects TDP-43 inclusions in human brain tissue with TDP-43 proteinopathy, but does not detect full-length nuclear TDP-43.
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide C-terminal to the caspase-cleavage site (between D219 and V220) of human TDP-43.
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot dependent
Purification	Affinity purification
Storage Buffer	PBS + 30% glycerol
Storage Condition	Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Target
Gene ID	23435
Swiss Prot	Q13148
Synonyms	ALS10 antibody, OTTHUMP00000002171 antibody, OTTHUMP00000002172 antibody, OTTHUMP00000002173 antibody, TADBP_HUMAN antibody, TAR DNA binding protein 43 antibody, TAR DNA binding protein antibody, TAR DNA-binding protein 43 antibody, TARDBP antibody, TDP 43 antibody, TDP-43 antibody, TDP43 antibody
Background	TDP43 (Tar DNA Binding 43, TARDBP) was originally identified as a protein which binds to the "transactivation response" (TAR) sequence found in the long terminal repeat of the HIV-1 virus genome (Ou et al.,1995). UV cross-linking of HeLa cell extract revealed a 43kDa protein which was cloned and sequenced and shown to contain two copies of the ~90 amino acid RRM domain. RRM is an acronym for RNA Recognition Motif, and this domain is found in many proteins which bind single stranded RNA and some which bind single stranded DNA. Northern blots showed that the protein is ubiquitous in tissue expression. Much interest has been focused on TDP43 recently due to its association with the inclusions seen in frontotemporal lobar degeneration and Amyotrophic Lateral Sclerosis (Neumann et al., 2006). The protein is present in these inclusions in partially degraded, hyperphosphorylated and ubiquitinated forms.
Application Dilution
WB	1:300-5000
IHC