MEK1/2 (Ser218,222) Antibody

Applications

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Bovine
Chicken
Zebrafish
Xenopus
Non-Human Primate

Overview
Catalog #	bs-70152r-100ul
Product Name	MEK1/2 (Ser218,222) Antibody
Applications	WB
Specificity	Specific for endogenous levels of the ~45 kDa MEK 1/2 protein phosphorylated at Ser218/222. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide. Note: The phosphosites in human MEK 1/2 are Ser218 and Ser222; the phosphosites in mouse MEK 1/2 are Ser217 and Ser221.
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Bovine, Chicken, Zebrafish, Xenopus, Non-Human Primate
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser218/222 of human MEK1/2, conjugated to keyhole limpet hemocyanin (KLH).
Modification Site	Ser218,222
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot dependent
Purification	Antigen Affinity purification from Pooled whole antiserum
Storage Buffer	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Gene ID	5604
Swiss Prot	Q02750
Synonyms	AA589381 antibody, CFC3 antibody, Dual specificity mitogen-activated protein kinase kinase 1 antibody, Dual specificity mitogen-activated protein kinase kinase 2 antibody, EC 2.7.12.2 antibody, ERK activator kinase 1 antibody, ERK activator kinase 2 antibody, FLJ26075 antibody, MAP kinase kinase 1 antibody, MAP kinase kinase 2 antibody, MAP2K1 antibody, MAP2K2 antibody, MAPK/ERK kinase 1 antibody, MAPK/ERK kinase 2 antibody, MAPKK 1 antibody, MAPKK1 antibody, MAPKK2 antibody, MEK 1 antibody, MEK1 antibody, MEKK1 antibody, Mitogen activated protein kinase kinase 1 antibody, Mitogen activated protein kinase kinase 2 antibody, Mitogen-activated protein kinase kinase 2, p45 antibody, MK2 antibody, MKK 1 antibody, MKK 2 antibody, MKK1 antibody, MKK2 antibody, MP2K1_HUMAN antibody, PRKMK 1 antibody, PRKMK 2 antibody, Prkmk1 antibody, Prkmk2 antibody, protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) antibody, Protein kinase, mitogen-activated, kinase 1 antibody, Protein kinase, mitogen-activated, kinase 2 antibody
Background	MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992).
Application Dilution
WB	1:300-5000