GABAA Receptor δ, N-Terminus Antibody

Applications

Reactivity

Mouse
Rat

Overview
Catalog #	bs-70107r-100ul
Product Name	GABAA Receptor δ, N-Terminus Antibody
Applications	WB, IHC
Specificity	Specific for endogenous levels of the ~50 kDa δ-subunit of the GABAA receptor.
Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Fusion protein from the N-terminus of the δ subunit of rat GABAA receptor.
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot Dependent
Purification	Antigen Affinity purification from Pooled whole antiserum
Storage Buffer	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Gene ID	29689
Swiss Prot	P18506
Synonyms	GABA(A) receptor subunit delta antibody, Gabrd antibody, Gamma aminobutyric acid GABA A receptor delta antibody, Gamma aminobutyric acid receptor delta subunit precursor GABA A receptor antibody, Gamma-aminobutyric acid receptor subunit delta antibody, GBRD_HUMAN antibody, MGC45284 antibody
Background	Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl– channel associated with the GABA-A receptor (GABA-A-R) subtype. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin,1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, co-expression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different a-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003). More recently there have been a number of studies demonstrating that the δ-subunit of the receptor may affect subunit assembly (Korpi et al., 2002) and may also confer differential sensitivity to neurosteroids and to ethanol (Wallner et al., 2003; Wohlfarth et al., 2002).
Application Dilution
WB	1:300-5000
IHC