Fractin Antibody

Applications

Reactivity

Human
Rat

Overview
Catalog #	bs-70093r-100ul
Product Name	Fractin Antibody
Applications	WB, IHC
Specificity	Specific for endogenous levels of the ~32 kDa fractin protein. Shows no reactivity toward intact actin. There is often a ladder of smaller bands in cells or culture or in vivo preparations due to further degradation by other proteases. In IHC the antibody detects the processes and cell bodies of degenerating neurons and plaque-associated microglia in Alzheimer's disease.
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide corresponding to amino acid residues from the C-terminal region of the 32 kDa actin fragment.
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot Dependent
Purification	Neat whole antiserum
Storage Buffer	Neat whole antiserum
Storage Condition	Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Target
Synonyms	Fragment of actin, actin cleavage product
Background	Fractin (fragment of actin) is a caspase-specific cleavage product of actin and serves as a novel marker of apoptosis-related events. The antibody has been shown to detect the processes and cell bodies of degenerating neurons and plaque-associated microglia in Alzheimer’s disease (Yang et al., 1998). It has recently been reported that Fractin may have a functional role in apoptotic signaling in oligodendrocytes (Schulz, R., et al., Glia, 2009, in press).
Application Dilution
WB	1:300-5000
IHC