| Overview |
| bs-70081r-100ul |
| EphrinB (Tyr317) Antibody |
| WB |
| Specific for endogenous levels of the ~46 kDa EphrinB protein phosphorylated at Tyr317. Immunolabeling is completely eliminated by treatment with λ-phosphatase. Note: Chicken Tyr317 is the homolog of rat and mouse Tyr328, human Tyr329 and Xenopus Tyr310. |
| Mouse, Rat |
| Human, Bovine, Chicken, Zebrafish, Xenopus |
| Specifications |
| Unconjugated |
| Rabbit |
| Synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho Tyr317 of chicken EphrinB, conjugated to keyhole limpet hemocyanin (KLH). |
| Tyr317 |
| Polyclonal |
| IgG |
| Lot dependent |
| Antigen Affinity purification from Pooled whole antiserum |
| 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target |
| 396513 |
| P28693 |
| CEK5 ligand antibody, EFNB1 antibody, ELK L antibody, ELK ligand antibody, ELKL antibody, EPH related receptor tyrosine kinase ligand 2 antibody, Ephrin B1 antibody, Ephrin B2 antibody, LERK2 antibody |
| EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vanderhaeghen, 1998; Dufour et al., 2003; Oike et al., 2002). EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr-298) plays a key role in EphrinB signaling (Kalo, et al., 2001). |
| Application Dilution |
| WB |
1:300-5000 |
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