| Overview |
| bs-70057R |
| CtIP (Thr847) Antibody |
| WB |
| Specific for endogenous levels of the ~100 kDa CtIP protein phosphorylated at Thr847. Immunolabeling is completely eliminated by treatment with λ-phosphatase. |
| Human |
| Mouse, Dog, Bovine, Sheep, Chicken, Guinea Pig, Goat, Non-Human Primate, Finch |
| Specifications |
| Unconjugated |
| Rabbit |
| Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr847 of human CtIP, conjugated to keyhole limpet hemocyanin (KLH). |
| Thr847 |
| Polyclonal |
| IgG |
| Lot Dependent |
| Antigen Affinity purification from Pooled whole antiserum |
| 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target |
| 5932 |
| Q99708 |
| COM1 antibody, COM1_HUMAN antibody, CtBP interacting protein antibody, CtBP-interacting protein antibody, CtIP antibody, DNA endonuclease RBBP8 antibody, JWDS antibody, RB binding protein 8 endonuclease antibody, RBBP-8 antibody, RBBP8 antibody, Retinoblastoma-binding protein 8 antibody, Retinoblastoma-interacting protein and myosin-like antibody, Rim antibody, SAE2 antibody, SCKL2 antibody, Sporulation in the absence of SPO11 protein 2 homolog antibody |
| CtIP, C-terminal binding protein-interacting protein, is a DNA endonuclease activated by double stranded breaks (DSBs). DSB repairs can be performed by either one of two mechanisms; non-homologous end joining (NHEJ) or homologous recombination (HR). NHEJ is the predominant DSB repair pathway throughout the entire cell cycle, most importantly in the G1 phase (Rothkamm et al, 2003); while HR is important for repairing DSBs in S and G2 phases (Beucher et al, 2009). CtIP controls DSB resection; an event that only occurs in HR during G2-phase. Phosphorylation of Thr-847 dictates the resection efficiency (Huertas et al, 2008). Furthermore, it has been found that DSBs undergo resection and repair in G1-phase cells via a process requiring Plk3 phosphorylation of CtIP at Ser-327 and Thr-847 (Barton et al, 2014). Several additional phosphorylation sites within CtIP have been identified, but their significance in the repair of DNA have yet to be determined. |
| Application Dilution |
| WB |
1:300-5000 |
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