ATF2 (Thr52) Antibody

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bs-70023r-100ul
Product Name	ATF2 (Thr52) Antibody
Applications	WB
Specificity	Specific for endogenous levels of the ~72 kDa ATF2 protein phosphorylated at Thr52. Immunolabeling of the ATF2 band is reduced by treatment of the cells with the PKC catalytic inhibitor Gö6850.
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr52 of human ATF2, conjugated to keyhole limpet hemocyanin (KLH).
Modification Site	Thr52
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot Dependent
Purification	Antigen Affinity purification from Pooled whole antiserum
Storage Buffer	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Gene ID	1386
Swiss Prot	P15336
Synonyms	Activating transcription factor 2 antibody, Activating transcription factor 2 splice variant ATF2 var2 antibody, ATF 2 antibody, Atf-2 antibody, Atf2 antibody, ATF2 protein antibody, ATF2_HUMAN antibody, cAMP Response Element Binding Protein 2 antibody, cAMP response element binding protein CRE BP1 antibody, cAMP response element-binding protein CRE-BP1 antibody, cAMP responsive element binding protein 2, formerly antibody, cAMP-dependent transcription factor ATF-2 antibody, cAMP-responsive element-binding protein 2 antibody, CRE BP1 antibody, CRE-BP antibody, CREB 2 antibody, CREB-2 antibody, CREB2 antibody, CREBP1 antibody, Cyclic AMP dependent transcription factor ATF 2 antibody, Cyclic AMP-dependent transcription factor ATF-2 antibody, Cyclic AMP-responsive element-binding protein 2 antibody, D130078H02Rik antibody, D18875 antibody, HB 16 antibody, HB16 antibody, Histone acetyltransferase ATF2 antibody, MGC105211 antibody, MGC105222 antibody, MGC111558 antibody, MGC142504 antibody, mXBP antibody, MXBP protein antibody, Tg(Gzma-Klra1)7Wum antibody, TREB 7 antibody, TREB7 antibody
Background	The transcription factor ATF2 is a member of the ATF/CREB family of leucine zipper proteins. In response to stress stimuli, it activates a variety of gene targets that are involved in oncogenesis, and has been correlated with maintenance of a cancer cell phenotype (Vlahopoulos et al., 2008). Inhibiting ATF2 impedes melanoma development and elicits tumor suppressor function (Bhoumik et al., 2008). To act as a tumor suppressor, ATF2 must localize at the mitochondria, and phosphorylation at Thr-52 by PKCε regulates this translocation (Lau et al., 2012).
Application Dilution
WB	1:300-5000