5-Lipoxygenase (Ser523) Antibody

Applications

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Non-Human Primate

Overview
Catalog #	bs-70002r-100ul
Product Name	5-Lipoxygenase (Ser523) Antibody
Applications	WB
Specificity	Specific for endogenous levels of the ~80 kD 5-LO phosphorylated at Ser523 in Western blots. Immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-phosphatase, 1200 units for 30 min).
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Non-Human Primate
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser523 of human 5-lipoxygenase (5-LO), conjugated to keyhole limpet hemocyanin (KLH).
Modification Site	Ser523
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot Dependent
Purification	Antigen Affinity purification from Pooled whole antiserum
Storage Buffer	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Gene ID	240
Swiss Prot	P09917
Synonyms	5 Lipoxygenase antibody, 5 LO antibody, 5 LOX antibody, 5-lipoxygenase antibody, 5-LO antibody, 5-LOX antibody, 5LOX antibody, 5LPG antibody, ALOX 5 antibody, Alox5 antibody, Arachidonate 5 lipoxygenase antibody, Arachidonate 5-lipoxygenase antibody, arachidonic 5-lipoxygenase alpha-10 isoform antibody, arachidonic 5-lipoxygenase delta-10-13 isoform antibody, arachidonic 5-lipoxygenase delta-13 isoform antibody, arachidonic 5-lipoxygenase delta-p10 isoform antibody, Arachidonic acid 5 lipoxygenase antibody, Leukotriene A4 synthase antibody, LOG 5 antibody, LOG5 antibody, LOX5_HUMAN antibody, MGC163204 antibody
Background	The enzyme 5-lipoxygenase (5-LO) plays a key role in regulating the production of leukotrienes (LTs) (Funk, 2001). Overproduction of LTs contributes to several diseases, most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994), fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004). Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine-523 in 5-LO (Luo et al., 2004). Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, then, could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to Ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis and atherosclerosis.
Application Dilution
WB	1:300-5000