Tartrate Resistant Acid Phosphatase Polyclonal Antibody

Molt-4 cell lysates probed with Tartrate Resistant Acid Phosphatase Polyclonal Antibody, Unconjugated (bs-6434R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse Liver; Probed with Tartrate Resistant Acid Phosphatase Polyclonal Antibody, Unconjugated (bs-6434R) at 1:300, overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Liver; Probed with Tartrate Resistant Acid Phosphatase Polyclonal Antibody, Unconjugated (bs-6434R) at 1:300, overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$A431 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with ACP5 Antibody(bs-6434R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).$

A431 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with ACP5 Antibody(bs-6434R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Applications

WB
ELISA
FCM

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Pig
Horse
Rabbit

Overview
Catalog #	bs-6434R
Product Name	Tartrate Resistant Acid Phosphatase Polyclonal Antibody
Applications	WB, ELISA, FCM
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Tartrate-resistant acid phosphatase type 5
Immunogen Range	101-200/325
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	54
Swiss Prot	P13686
Subcellular location	Cytoplasm
Synonyms	TRAP; SPENCDI; Tartrate-resistant acid phosphatase type 5; TR-AP; Tartrate-resistant acid ATPase; TrATPase; Type 5 acid phosphatase; ACP5; TRACP5a; TRACP5b
Background	Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100