DAL1/EPB41L3 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with DAL1 Polyclonal Antibody, Unconjugated (bs-6003R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with DAL1 Polyclonal Antibody, Unconjugated (bs-6003R) at 1:400 overnight at 4\u00b0C, DAB staining.

$Lane 1: Human HUVEC cell lysates; Lane 2: Human U-2 OS cell lysates probed with DAL1\/EPB41L3 Polyclonal Antibody, Unconjugated (bs-6003R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human HUVEC cell lysates; Lane 2: Human U-2 OS cell lysates probed with DAL1\/EPB41L3 Polyclonal Antibody, Unconjugated (bs-6003R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-6003R
Product Name	DAL1/EPB41L3 Polyclonal Antibody
Applications	WB, ELISA
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human DAL1/EPB41L3
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23136
Swiss Prot	Q9Y2J2
Subcellular location	Cell membrane
Synonyms	Differentially expressed in adenocarcinoma of the lung protein 1; 4.1B; Band 4.1 like protein 3; DAL 1; DAL1; EPB41L3; Erythrocyte membrane protein band 4.1 like 3; HGNC:3380; KIAA0987; E41L3_HUMAN.
Application Dilution
WB	=1:500-2000
ELISA	ELISA=1:5000-10000