Centaurin gamma 1 Polyclonal Antibody

Lane 1: Hela cell lysates probed with Centaurin gamma 1 Polyclonal Antibody, Unconjugated (bs-5930R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Pig
Horse

Overview
Catalog #	bs-5930R
Product Name	Centaurin gamma 1 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Centaurin gamma 1
Immunogen Range	981-1080/1192
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location	Cytoplasm, Nucleus
Synonyms	Centaurin gamma 1; ANK repeat and PH domain-containing protein 2; AGAP-2; Agap2; Agap2 ArfGAP with GTPase domain, ankyrin repeat and PH domain 2; AGAP2_HUMAN; ANK repeat and PH domains 2; Arf GAP with GTP binding protein like; Arf GAP with GTP-binding protein-like, ANK repeat and PH domains 2; Arf GAP with GTP-binding protein-like, ankyrin repeat and pleckstrin homology domains 2; Arf-GAP with GTPase; Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2; ArfGAP with GTPase domain, ankyrin repeat and PH domain 2; centaurin gamma1; Centaurin-gamma-1; Centg1; cnt g1; Cnt-g1; Cnt2; GGAP2; GTP binding and GTPase activating protein 2; GTP-binding and GTPase activating protein 2; GTP-binding and GTPase-activating protein 2; Nuclear GTPase PIKE; Phosphatidylinositol-3-kinase enhancer; Phosphoinositide 3 kinase enhancer; PI3K ENHANCER; PIKE.
Background	GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200