Integrin Alpha V + Beta 6 Antibody

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-Integrin Alpha V + Beta 6 Polyclonal Antibody, Unconjugated (bs-5791R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Hela cells probed with Integrin Alpha V + Beta 6 Antibody, Unconjugated (bs-5791R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Mouse kidney lysates probed with Integrin Alpha V + Beta 6 Polyclonal Antibody, Unconjugated (bs-5791R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Siha cell lysates probed with Integrin Alpha V + Beta 6 Polyclonal Antibody, Unconjugated (bs-5791R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-5791R
Product Name	Integrin Alpha V + Beta 6 Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Integrin Alpha V + Beta 6
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3685 3694
Subcellular location	Cell membrane
Synonyms	CD51; Integrin alpha-V light chain; integrin, alpha V vitronectin receptor, alpha polypeptide, antigen CD51; Integrin, alpha V; Integrin, beta 6; ITAV_HUMAN; ITGAV; ITGB6; Vitronectin receptor subunit alpha.
Background	Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin alpha-v beta-6 is upregulated in cancers and during tissue remodelling but is absent from resting adult tissues. Integrin alpha-v beta-6 promotes invasion and correlates with poor survival and therefore makes a promising therapeutic target.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200