XPA Polyclonal Antibody

Lane 1: Hela cell lysates; Lane 2: Jurkat cell lysates; Lane 3: K562 cell lysates; Lane 4: Mouse Liver lysates; Lane 5: Mouse Kidney lysates probed with XPA Polyclonal Antibody, Unconjugated (bs-55224R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Hela cell lysates; Lane 2: XPA knockout (KO) HeLa cell lysates probed with XPA Polyclonal Antibody, Unconjugated (bs-55224R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (XPA) polyclonal Antibody, Unconjugated (bs-55224R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes.Green\uff1aGFP-RNF168 fusion protein expression for DNA damage marker.DAPI (blue, C02-04002) was used to stain the cell nuclei.$

U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (XPA) polyclonal Antibody, Unconjugated (bs-55224R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes.Green\uff1aGFP-RNF168 fusion protein expression for DNA damage marker.DAPI (blue, C02-04002) was used to stain the cell nuclei.

$A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (XPA ) polyclonal Antibody, Unconjugated (bs-55224R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes.$

A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (XPA ) polyclonal Antibody, Unconjugated (bs-55224R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes.

$Lane 1: Human 293T cell lysates; Lane 2: Human HeLa cell lysates probed with XPA Polyclonal Antibody, Unconjugated (bs-55224R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human 293T cell lysates; Lane 2: Human HeLa cell lysates probed with XPA Polyclonal Antibody, Unconjugated (bs-55224R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IF(ICC)
IF

Reactivity

Human
Mouse

Overview
Catalog #	bs-55224R
Product Name	XPA Polyclonal Antibody
Applications	WB, IHC-P, IF(ICC), IF
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human XPA (NP_000371.1).
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	7507
Swiss Prot	P23025
Synonyms	XPA;XP1;XPAC
Background	This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. Alternatively spliced transcript variants have been found for this gene.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200
IF

Applications

Reactivity

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